KPV peptide tshuaj tsuagyog kev npaj qhov ncauj lossis qhov ntswg nrog ntuj tripeptide (lysine proline valine) ua cov ntsiab lus tseem ceeb. Nws qhia tau hais tias qhov zoo tshwj xeeb hauv kev kho cov hnyuv, kev tiv thaiv kab mob thiab kev tiv thaiv neuroprotection los ntawm kev tsom mus rau kev tswj cov teeb liab kab mob thiab txhim kho cov ntaub so ntswg ua haujlwm. Raws li ib feem ntawm alpha melanocyte stimulating hormone (alpha MSH), nws khaws nws cov khoom tiv thaiv - mob thaum tsis txhob muaj kev phiv xws li cov tshuaj hormone pigmentation, muaj kev nyab xeeb siab thiab ua haujlwm zoo. Cov khoom no muaj cov khoom xyaw yooj yim thiab tsis muaj cov tshuaj ua kom khaus. Nws tuaj yeem siv ib leeg lossis ua ke nrog kev kho peptides xws li BPC-157 los ua ib qho kev sib koom ua ke ntawm "anti-inflammatory+repair". Nws txoj kev tswj hwm ntawm qhov mob, kev txhawb nqa rau kev kho cov ntaub so ntswg, thiab kev xa khoom hla kev ua rau nws muaj peev xwm ua tau rau hauv kev ua haujlwm ntawm kev mob ntev, plab hnyuv, thiab neuroprotection.
Peb cov khoom los ntawm
KPV COA
Antimicrobial:
Ua txhaum los ntawm qhov teeb meem ntawm kev siv tshuaj tiv thaiv - dual tiv thaiv kab mob mechanism thiab kev kho mob txhais tau tias muaj peev xwm
Tawm tsam keeb kwm yav dhau los ntawm cov tshuaj tua kab mob ua rau muaj teeb meem kev noj qab haus huv thoob ntiaj teb,KPV peptide tshuaj tsuagmuab cov tswv yim tshiab los daws cov tshuaj - cov kab mob tiv thaiv kab mob los ntawm nws qhov tshwj xeeb dual cov tshuaj tiv thaiv kab mob - ncaj qha tua cov kab mob thiab kev tswj hwm kev tiv thaiv kab mob. Nws cov txheej txheem ntawm kev ua haujlwm npog peb hom kab mob loj: cov kab mob, cov kab mob, thiab cov kab mob, thiab txo cov kev pheej hmoo ntawm kev siv tshuaj tiv thaiv los ntawm ntau yam - phiaj xwm kev cuam tshuam, ua rau pom qhov tseem ceeb ntawm kev siv tshuaj kho mob.
Nws muaj cov nyhuv inhibitory zoo ntawm cov kab mob Gram zoo (xws li Staphylococcus aureus) thiab qee cov kab mob Gram tsis zoo (xws li Pseudomonas aeruginosa). Nws lub ntsiab mechanism muaj xws li:
Biofilm cuam tshuam: Staphylococcus aureus txhim kho cov tshuaj tiv thaiv los ntawm kev tsim biofilms, uas tuaj yeem degrade polysaccharide protein complexes hauv biofilm matrix, nthuav tawm cov kab mob los tiv thaiv kab mob thiab tshuaj tua kab mob. Qhov kev sim tau pom tias tom qab kev kho KPV, biofilm tuab tau txo qis los ntawm 70% thiab cov kab mob sib kis tau nce peb zaug.
Inhibition ntawm virulence yam tseem ceeb: Los ntawm kev txo qis kev qhia ntawm qhov tseem ceeb virulence yam xws li alpha hemolysin (Hla) thiab Panton Valentine leukopenin (PVL), cov kab mob pathogenicity txo qis. Piv txwv li, nyob rau hauv tus qauv kev kub hnyiab, kev sib xyaw ua ke ntawm vancomycin nce cov kab mob tshem tawm los ntawm 65% thaum siv ib leeg mus rau 95%, thaum txo cov tshuaj lom cov ntaub so ntswg.
Kev tiv thaiv kab mob tiv thaiv kab mob: tsis ua rau cov kab mob ib txwm muaj xws li cell phab ntsa synthesis lossis DNA replication, tab sis inhibits cov kab mob sib koom ua ke los ntawm kev cuam tshuam nrog quorum sensing systems, yog li txo qhov siab ntawm cov tshuaj tiv thaiv kab mob sib hloov. Nws tus nqi MIC (qhov tsawg kawg nkaus inhibitory concentration) yog 16 μ g / mL, thiab nws muaj cov nyhuv synergistic thaum siv ua ke nrog vancomycin.
Candida albicans yog cov kab mob tseem ceeb ua rau lub tsev kho mob kis tau kab mob, thiab nws cov biofilm tsim thiab fluconazole kuj ua rau kev kho mob nyuaj. Ua si cov teebmeem antifungal los ntawm cov txheej txheem hauv qab no:
Cell membrane cuam tshuam: Nws tuaj yeem nkag mus rau hauv phospholipid bilayer ntawm fungal cell membranes, tsim cov pores uas ua rau kom cov ntsiab lus ntawm tes. Qhov kev sim tau pom tias qhov nce ntawm propidium iodide (PI) los ntawm Candida albicans tau nce plaub npaug tom qab kev kho mob, qhia tias qhov poob ntawm daim nyias nyias.
Reversal of drug-resistant strains: Fluconazole resistant strains acquire resistance by overexpressing efflux pumps (such as CDR1, MDR1) or target enzyme (ERG11) mutations, and by inhibiting efflux pump function and interfering with ergosterol synthesis, the MIC value of the resistant strain decreases from>64 μg / ml rau 32 μg / ml.
Biofilm inhibition: Nws tuaj yeem thaiv qhov kev qhia ntawm cov noob tseem ceeb (xws li HWP1 thiab ALS3) koom nrog kev tsim thiab kev loj hlob ntawm Candida albicans hyphae, uas ua rau 65% txo qis hauv biofilm biomass.
KPV peptide tshuaj tsuagexerts ib tug tiv thaiv kab mob nyob rau hauv tus kab mob los ntawm lub hom phiaj tus kab mob lifecycle thiab host inflammatory teb:
HSV-1 inhibition: Nws tuaj yeem khi rau lub hnab ntawv glycoprotein D (gD) ntawm tus kab mob herpes simplex virus (HSV-1), thaiv kev sib xyaw ntawm tus kab mob nrog tus tswv tsev cell membrane, thiab nres cov kab mob rov ua dua. Hauv cov qauv kab mob pob txha, cov kab mob kis tau raug txo los ntawm 4 logarithmic xaj ntawm qhov loj (104 CFU / mL → 100 CFU / mL), thiab pob txha opacity thiab neovascularization raug txo.
Kev tswj cov cua daj cua dub: Cov kab mob kis feem ntau ua rau muaj cua daj cua dub (xws li kev tso tawm ntau dhau ntawm IL-6 thiab TNF - ), uas txo cov ntaub so ntswg puas tsuaj los ntawm inhibiting NF - κ B txoj hauv kev thiab NLRP3 inflammasome activation, uas ua rau 70% txo qis hauv cytokine.
Muaj peev xwm tiv thaiv kab mob dav dav: Cov kev tshawb fawb ua ntej tau pom tias KPV kuj muaj qhov cuam tshuam rau cov kab mob pseudovirus qauv ntawm cov kab mob ua pa syncytial (RSV) thiab tus mob coronaviruses (xws li SARS-CoV-2), qhia nws lub peev xwm ua tus neeg sib tw molecule rau broad-spectrum antiviral therapy.
Cov khoom siv tshuaj tua kab mob tau raug lees paub hauv ntau qhov xwm txheej kho mob:
Cov kab mob mob ntev: mob ntshav qab zib ko taw rwj feem ntau cuam tshuam nrog ntau cov tshuaj tiv thaiv kab mob (xws li MRSA) kab mob, thiab kev kho tus nqi ntawm kev kho ib txwm muaj tsawg dua 30%. Los ntawm kev tso tawm tsis tu ncua ntawm peptide molecules, hydrogel tau nce qhov kev tshem tawm ntawm MRSA mus rau 82%, txo lub sijhawm kho los ntawm qhov nruab nrab ntawm 28 hnub mus rau 12 hnub, thiab tsis muaj cov ntaub ntawv qhia txog tshuaj tiv thaiv.
Lub tsev kho mob kis tau mob ntsws: Cov neeg mob tau txais cov tshuab ua pa tuaj yeem ua rau muaj ntau yam tshuaj - tiv thaiv kab mob ntsws (xws li Acinetobacter baumannii). Nebulization inhalation ua ke nrog kev kho tshuaj tua kab mob txo cov tshuab ua pa los ntawm 4.2 hnub, txo ICU nyob ntawm 6 hnub, thiab txo qhov kev pheej hmoo ntawm cov kab mob fungal thib ob.
Biofilm txuam nrog kab mob: Hauv cov qauv kev cog hniav orthopedic, kev txhaj tshuaj hauv zos txo qis biofilm los ntawm 85% mus rau 15% thiab txhawb kev sib koom ua ke ntawm pob txha, muab txoj hauv kev tshiab rau kev cog qoob loo khaws cia.
Antitumor:
Los ntawm Inhibition mus rau Immune Activation - Multi pathway Antitumor Mechanisms and Combination Therapy Strategies
Cov tshuaj tiv thaiv - cov nyhuv qog tawg los ntawm tib hom cytotoxic ntawm cov tshuaj khomob ib txwm muaj, tsim cov nyhuv synergistic los ntawm peb lub tswv yim ntawm kev cuam tshuam ncaj qha ntawm cov qog hlwb, tiv thaiv angiogenesis, thiab kev tiv thaiv kab mob, thiab ua kom pom qhov zoo ntawm kev txhim kho kev ua tau zoo thiab txo cov tshuaj lom hauv kev sib xyaw ua ke.
Kev cuam tshuam ntawm cov qog cell ciaj sia los ntawm ntau- lub hom phiaj mus kom ze:
Inhibition ntawm Wnt/ - catenin txoj hauv kev: Txoj kev no yog qhov txawv txav hauv cov qog nqaij hlav xws li mob qog noj ntshav thiab mob qog noj ntshav, thiab ua rau apoptosis ntawm HCT116 cov kab mob qog nqaij hlav hauv plab los ntawm kev txhawb nqa - catenin degradation thiab downregulating downstream gene qhia (xws li kuv cov kab mob hauv C{3} thiab C{3} inhibition tus nqi ntawm 68%.
EMT cov txheej txheem thaiv: Epithelial mesenchymal hloov pauv (EMT) yog ib kauj ruam tseem ceeb hauv qog migration, uas tuaj yeem txhim kho E-cadherin qhia thiab txo qis N-cadherin thiab vimmentin, txo cov qog cell tsiv teb tsaws los ntawm 50%.
Kev cuam tshuam metabolic reprogramming: Los ntawm inhibiting lactate dehydrogenase A (LDHA) kev ua haujlwm, txo cov khoom siv hluav taws xob nyob ntawm Warburg cov nyhuv ntawm cov qog hlwb, cov qog loj hlob yog txo los ntawm 40%.
qog neovascularization yog lub hauv paus ntawm cov qog loj hlob thiab metastasis, exerting anti angiogenic los ntawm cov txheej txheem hauv qab no:
VEGF teeb liab inhibition: Nws tuaj yeem khi rau vascular endothelial growth factor (VEGF) receptor 2 (VEGFR2), thaiv VEGF-VEGFR2 khi thiab downstream Akt/mTOR txoj kev ua kom, ua rau 75% inhibition tus nqi ntawm endothelial cell proliferation.
Vascular normalization: Tsis zoo li cov tshuaj tiv thaiv angiogenic, nws tuaj yeem cuam tshuam qhov txawv txav ntawm angiogenesis thaum txhawb kev loj hlob ntawm cov hlab ntsha seem, txhim kho cov qog nqaij hlav oxygenation thiab kev xa tshuaj. Hauv cov qauv mob qog noj ntshav, KPV kev kho mob txo cov qog microvessel ceev los ntawm 52%, thiab qhov concentration ntawm cov tshuaj kho mob (xws li paclitaxel) hauv cov qog tau nce 2.3 zaug.
3. Kev tiv thaiv kab mob: thim rov qab cov qog tiv thaiv kab mob
Microenvironment
Reshaping lub qog tiv thaiv kab mob los ntawm kev tswj lub cev tiv thaiv kab mob ua haujlwm:
DC cell maturation nce qib:KPV peptide tshuaj tsuagtuaj yeem txhim kho qhov kev qhia ntawm co stimulatory molecules (CD80, CD86) thiab MHC-II nyob rau saum npoo ntawm cov hlwb dendritic (DCs), txhim kho lawv cov peev xwm nthuav tawm antigen. Hauv cov qauv melanoma, qhov kev faib ua feem ntawm cov laus DCs hauv cov qog qog ntshav ntawm cov qog tau nce los ntawm 15% mus rau 42%.
Txhim kho CD8+T cell infiltration: Los ntawm inhibiting CXCL12/CXCR4 axis, tiv thaiv kev tiv thaiv Treg cell infiltration yog txo, thaum txhawb CXCL9/CXCL10 secretion los nrhiav CD8+T cells. Cov kev sim tau pom tias kev kho mob nce cov qog infiltrating lymphocytes (TILs) los ntawm 3.5 npaug, nrog rau qhov feem ntawm CD8+T hlwb nce los ntawm 12% mus rau 35%.
Kev tsim lub cev tiv thaiv kab mob: tuaj yeem ua rau muaj qhov sib txawv ntawm lub hauv paus nco T-hlwb (Tcm), txo cov qog rov qab los ntawm 60%, qhia tias nws muaj peev xwm siv tau hauv kev kho mob tom qab phais.
4. Kev sib xyaw ua ke: kev kho mob zoo ntawm kev txhim kho kev ua tau zoo thiab txo cov toxicity
Kev sib xyaw ua ke ntawm KPV thiab cov tshuaj tiv thaiv tam sim no -cov qog nqaij hlav tuaj yeem txhim kho kev ua tau zoo thiab txo cov kev mob tshwm sim:
KPV + chemotherapy: Hauv cov qauv mob qog noj ntshav, kev sib xyaw ua ke ntawm cisplatin txo cov qog ntim los ntawm 89% (62% hauv pawg cisplatin monotherapy), thiab qhov tshwm sim ntawm cov pob txha pob txha txo qis los ntawm 55% mus rau 40%. Cov txheej txheem muaj feem xyuam nrog KPV kev tiv thaiv ntawm hematopoietic qia hlwb thiab inhibition ntawm inflammatory yam xws li IL-6.
KPV + cov tshuaj tiv thaiv kab mob tiv thaiv kab mob: PD-1/PD-L1 cov tshuaj tiv thaiv kab mob muaj cov lus teb tsawg dua 20% hauv "cov qog mob khaub thuas" (xws li mob qog noj ntshav), thaum KPV nce PD-1 cov tshuaj tiv thaiv kab mob rau 65% thiab kev ciaj sia nruab nrab los ntawm kev thim rov qab Treg cell inhibition thiab nce P-L1.
KPV + xov tooj cua: tuaj yeem txhim kho hluav taws xob-induced immunogenic cell death (ICD), nce tus nqi txo ntawm cov kab mob unirradiated nyob deb ("cov nyhuv nyob deb") los ntawm 15% mus rau 38%, muab lub tswv yim tshiab rau cov qog nqaij hlav oligometastatic.
Raws li ib tug multifunctional peptide ntawm tej yam ntuj tso keeb kwm, nws antimicrobial thiab anti- qog mechanisms sib sib zog nqus nyob rau hauv txoj kab nrog unmetal kev kho mob xav tau kev pab: nyob rau hauv lub teb ntawm tus kab mob, nws dual mechanism lov los ntawm qhov teeb meem ntawm cov tshuaj tiv thaiv; Hauv kev ua haujlwm ntawm oncology, nws txoj kev sib koom ua ke los ntawm kev cuam tshuam ncaj qha rau kev tiv thaiv kab mob ua rau muaj kev sib koom tes zoo rau kev sib xyaw ua ke. Nrog rau kev ua kom zoo ntawm kev xa khoom thev naus laus zis xws li nanomaterials thiab cov phiaj xwm hloov kho, nws xav tias yuav dhau los ua cov khoom tseem ceeb ntawm cov tiam tom ntej ntawm kev tiv thaiv kab mob thiab tiv thaiv- kho mob qog, txhawb kev txhim kho cov tshuaj precision.
Cim npe nrov: Tuam Tshoj kpv peptide spray manufacturers, lwm tus neeg
