Ntses Ntses Collagen Powder

Cov yam ntxwv tseem ceeb ntawm daim tawv nqaij laus yog qhov poob thiab kev puas tsuaj ntawm collagen hauv txheej dermis. Collagen peptides tau dhau los ua cov khoom tseem ceeb rau kev hloov, ntxiv, thiab ua kom cov collagen synthesis endogenous vim lawv qhov hnyav molecular, siab biocompatibility, thiab yooj yim nqus los ntawm daim tawv nqaij.

Txawm li cas los xij, raws li qhov loj tshaj plaws hauv lub cev hauv tib neeg lub cev, "cib av" qauv ntawm daim tawv nqaij stratum corneum (tsim los ntawm keratinocytes li cib thiab intercellular lipids li av nkos) tsim ib qho nyuaj permeation barrier, ua rau nws nyuaj rau ntau tshaj 90% ntawm cov khoom xyaw hauv zos kom nkag mus rau stratum derum. Rau FCP, transdermal absorption ntsib ob qhov teeb meem: thawj zaug, qhov hnyav molecular feem ntau tshaj qhov nkag mus rau ntawm 500Da, uas yooj yim cuam tshuam los ntawm stratum corneum; Qhov thib ob, peptide chains muaj zog polarity thiab cov lipid solubility tsis zoo, ua rau nws nyuaj rau nkag mus rau lipid bilayer. Cov ntaub ntawv qhia tau hais tias tus transdermal absorption tus nqi ntawm qhov zoo tib yam FCP tshuaj pleev ib ce yog tsawg tshaj li 5%, thiab feem ntau ntawm cov khoom xyaw tsuas yog nyob rau saum npoo ntawm daim tawv nqaij kom muaj moisturizing los, tsis muaj peev xwm ua tau sib sib zog nqus los tiv thaiv - laus.

Lub transdermal efficiency ntawm FCP tsis yog txiav txim los ntawm ib qho tseem ceeb, tab sis, theej ib tug dynamic game txheej txheem nyob rau hauv uas molecular hnyav, qhov chaw, thiab matrix sib cuam tshuam thiab kev koom tes, tsim lub hauv paus ntsiab lus ntawm "kev nkag mus rau daim tawv nqaij":
Molecular hnyav: qhov 'lub cev qhov pib' uas txiav txim siab qhov tshwm sim ntawm permeation. Qhov me me ntawm qhov hnyav molecular, qhov yooj yim dua nws nkag mus, tab sis yog tias nws me dhau, nws yog qhov ua rau deactivation thiab tsis zoo stability;
Source: Lub hauv paus caj dab uas txiav txim siab cov qauv thiab kev ua haujlwm ntawm peptide chains. Cov peptide sequences thiab amino acid compositions ntawm txawv ntses qhov chaw sib txawv heev, ncaj qha cuam tshuam rau lawv affinity nrog daim tawv nqaij;

Lub ntsiab lus ntawm qhov kev ua si ntawm peb yog qhov sib npaug ntawm kev ua haujlwm ntawm permeation efficiency, lom neeg ua haujlwm, thiab ruaj khov: nrhiav qhov kawg me me molecular hnyav tuaj yeem ua rau poob ntawm kev ua haujlwm; Kev cia siab ib leeg rau siab - cov peev txheej zoo tsis tuaj yeem kov yeej cov teeb meem thiab kev txwv; Kev cia siab ib leeg ntawm kev ua kom zoo dua matrix tsis muaj kev txhawb nqa peptide saw. Yog li ntawd, kev tshuaj xyuas cov txheej txheem ywj pheej thiab kev sib koom tes kev ua si ntawm peb yog tus yuam sij rau kev tawg los ntawm FCP transdermal tshuaj xa lub raj mis caj dab thiab txhim kho cov qauv kev tu tawv nqaij, uas yog qhov tseem ceeb rau kev txhawb nqa kev tshawb fawb thiab kev txhim kho ntawm collagen peptide skincare.

Cov tawv nqaij yog tsim los ntawm peb txheej: epidermis, dermis, thiab cov ntaub so ntswg subcutaneous. Lub hauv paus tseem ceeb ntawm FCP transdermal xa tuaj los ntawm cov epidermis, tshwj xeeb tshaj yog cov stratum corneum.

Stratum corneum: Cov txheej txheej sab nraud ntawm cov epidermis, muaj li ntawm 15-20 txheej ntawm ca nucleated keratinocytes thiab intercellular lipids, nrog lub thickness ntawm kwv yees li 10-40 μ m. Keratin hlwb yog nplua nuj nyob rau hauv keratin, thiab intercellular lipids yog tsim los ntawm ceramides, cholesterol, thiab fatty acids nyob rau hauv ib tug ratio ntawm 1: 1: 1, tsim ib tug ntom lipid bilayer qauv, uas yog lub "thawj kab ntawm kev tiv thaiv" tiv thaiv infiltration ntawm lwm yam khoom.

Pob tshab txheej / granular txheej / spinous txheej / basal txheej: Hauv qab stratum corneum, muaj qhov nce hauv qhov chaw nruab nrab, qhov txo qis hauv cov ntsiab lus lipid, thiab txo qis hauv kev ua haujlwm. Cov txheej txheem basal yog txheej txheej ntawm cov qia cell, thiab FCP ncav cuag cov txheej txheem basal tuaj yeem ua kom cov qia cell sib txawv thiab txhawb nqa collagen synthesis.

Ntses Ntses Collagen Powdernkag mus rau ntawm daim tawv nqaij barrier tsuas yog los ntawm peb txoj kev, nrog rau kev txo qis kev ua haujlwm:

Txoj kev Interstitial: txoj kev tseem ceeb tshaj plaws, suav txog ntau dua 90% ntawm tag nrho cov ntim transdermal. FCP nkag mus rau lipid bilayer qhov sib txawv ntawm keratinocytes, nrog qhov sib txawv txoj kab uas hla ntawm 20-50nm, thiab yog cov tub ntxhais channel rau me me molecule peptide nkag.

Hla cellular txoj hauv kev: FCP ncaj qha nkag mus rau keratinocytes thiab yuav tsum tau dhau los ntawm cov cell membrane, cytoplasm, thiab nuclear membrane txuas ntxiv, nrog kev ua haujlwm zoo. Nws tsuas yog tsim nyog rau cov me me molecules uas tsis tshua muaj lipid solubility.

Txoj kev nkag mus: nkag mus los ntawm cov hauv paus plaub hau, hws qog, thiab cov qog sebaceous, suav txog tsawg dua 10%, tab sis tuaj yeem hla lub stratum corneum thiab ncaj qha mus txog cov txheej txheem dermis, ua lub luag haujlwm tseem ceeb hauv kev ntxiv rau kev nkag mus ntawm cov loj molecular peptides.

Xyoo 1990, Jan Bos thiab Marcus Meinardi tau tsim txoj cai 500 Dalton: cov khoom uas muaj qhov hnyav molecular tshaj 500 Da yog qhov nyuaj rau nkag mus rau stratum corneum ntawm daim tawv nqaij noj qab haus huv; Lub permeation efficiency ntawm cov tshuaj nrog ib tug molecular hnyav tsawg tshaj li 500Da ho ho nce raws li cov molecular hnyav. Cov txheej txheem tseem ceeb ntawm txoj cai no yog tias qhov chaw nruab nrab ntawm cov lipid bilayers ntawm stratum corneum yog txwv, thiab cov khoom loj ntawm cov khoom tsis tuaj yeem dhau los ntawm vim muaj kev cuam tshuam tsis zoo, thiab cov polar loj molecules nyuaj rau yaj hauv lipid txheej.

Rau FCP, txoj cai no yog qhov tseem ceeb nkag mus rau qhov txwv:

Molecular weight>3000Da: tsis tuaj yeem nkag mus rau hauv stratum corneum, tsuas yog tuaj yeem tsim cov yeeb yaj kiab moisturizing ntawm daim tawv nqaij;

1000-3000Da: ib qho me me ntawm kev nkag mus, feem ntau los ntawm txoj kev nkag, transdermal absorption tus nqi<5%;

500-1000Da: nruab nrab permeability, feem ntau los ntawm qhov chaw intercellular, transdermal haum tus nqi ntawm 5% -15%;

<500Da: Efficient penetration, can quickly penetrate the stratum corneum to reach the dermis layer, with a transdermal absorption rate of>20%.

Ntxiv rau qhov kev txwv qhov hnyav ntawm molecular, FCP transdermal kev xa tawm kuj tseem ntsib ob qhov teeb meem tseem ceeb, ntxiv rau qhov nyuaj ntawm qhov kev ua si:

Muaj zog polarity thiab tsis zoo lipid solubility: FCP muaj ntau ntawm hydrophilic amino thiab carboxyl pawg, nrog rau cov roj- dej faib coefficient (LogP)<-1, making it difficult to dissolve in the lipids of the stratum corneum and easily excluded from the lipid bilayer.

Peptide chains yog degradable yooj yim: Proteases muaj nyob rau ntawm daim tawv nqaij nto thiab stratum corneum, uas yuav degrade unprotected FCP, ua rau poob ntawm kev ua si; Meanwhile, me me molecule peptides (<300Da) have poor stability and are prone to aggregation and inactivation.

Lub ultra me me molecule FCP yog tsim los ntawm dipeptides thiab tripeptides (xws li Gly Pro, Gly Pro Hyp), nrog ib qho hnyav molecular concentrated ntawm 200-500Da, tag nrho ua tau raws li 500Da nkag mus rau qhov pib, thiab tuaj yeem nkag mus rau stratum corneum sai. Kev sim cov ntaub ntawv qhia tau hais tias lub<500Da ultra small molecule peptide derived from cod skin can achieve a transdermal absorption rate of 35% within 24 hours and reach the dermis layer within 1 hour.

Tab sis nws cov teeb meem tseem ceeb yog kev ua haujlwm tsis txaus thiab tsis muaj kev ruaj ntseg: luv peptide chains ua rau muaj pes tsawg leeg ntawm cov chaw nquag (Gly Pro Hyp, lub teeb liab tseem ceeb ntawm collagen synthesis), ua rau nws nyuaj rau kev ua haujlwm zoo fibroblasts; Lub caij no, lub polarity ntawm ultra me me molecule peptides muaj zog heev, ua rau lawv yooj yim rau diffuse thiab poob ntawm daim tawv nqaij nto, thiab yooj yim degraded los ntawm daim tawv nqaij proteases. Thaum siv tshuaj pleev ib leeg, qhov kev tuav pov hwm kev ua haujlwm yog tsawg dua 30%.

500-1000Da yog qhov zoo tshaj qhov hnyav molecular rau FCP, hu ua "golden permeation range". FCP nyob rau hauv ntau yam no feem ntau yog tsim los ntawm luv peptides ntawm 5-10 amino acids, uas muaj peb qhov zoo:

Kev nkag mus rau nruab nrab: nrog qhov hnyav ntawm molecular hauv qab 1000Da, nws tuaj yeem nkag mus los ntawm txoj hauv kev intercellular, nrog rau 24-teev transdermal absorption tus nqi kwv yees li 15%, ua tau raws li qhov yuav tsum tau ua;

Kev ua tau zoo tshaj plaws: muaj 3-5 Gly Pro Hyp nquag ua ntu zus, nws tuaj yeem khi zoo rau fibroblast nto receptors thiab qhib collagen synthesis signaling txoj hauv kev (xws li TGF - /Smad txoj kev);

Kev ruaj ntseg zoo: Cov saw peptide muaj qhov ntev me ntsis thiab tsis yooj yim sib sau los yog deactivated. Nws tuaj yeem nyob ruaj khov mus txog 6 lub hlis hauv cov tshuaj aqueous nrog rau kev ua haujlwm khaws cia ntau dua 80%.

Qhov nruab nrab molecular hnyavNtses Ntses Collagen Powdersaw caj dab yog qhov ntev thiab muaj ntau qhov chaw ua haujlwm, nrog rau kev ua haujlwm lom neeg zoo thiab ruaj khov. Txawm li cas los xij, nws raug txwv los ntawm txoj cai 500Da thiab nyuaj rau nkag mus rau stratum corneum. Nws qhov kev nkag mus tsuas yog nyob ntawm cov khoom siv xws li cov hauv paus plaub hau thiab cov qog hws, uas suav nrog tsawg dua 0.1% ntawm tag nrho cov tawv nqaij, ua rau 24-teev transdermal nqus tus nqi tsuas yog li 5%, nrog rau feem ntau cov khoom seem ntawm qhov chaw ntawm stratum corneum.

The core value of medium molecular weight peptides is moisturizing and surface repair: they can form a dense moisturizing film on the skin surface, lock in moisture, and improve dryness and roughness of the skin; Nyob rau tib lub sijhawm, nws tuaj yeem kho lipid kev puas tsuaj rau stratum corneum thiab txhim kho daim tawv nqaij ua haujlwm. Tab sis vim tsis muaj peev xwm ncav cuag lub dermis txheej, qhov sib sib zog nqus cuam tshuam xws li tiv thaiv -aging thiab firming tsis muaj zog.

Targeted enzymatic hydrolysis technology: precision txiav ntawm peptide chains

Siv cov khoom sib xyaw ua ke (trypsin + papain + elastase) es tsis txhob siv ib qho enzyme, los ntawm kev tswj cov enzymatic hydrolysis kub (40-50 degree), lub sij hawm (2-4 teev), thiab enzyme ntxiv npaum li cas (0.5% -1%), collagen peptide chains yog xaiv cleaved, ua rau ntau tshaj 70% ntawm peptide ntau yam ntawm peptide. Cov ntaub ntawv qhia tau hais tias qhov kev faib ua feem ntawm 500-1000Da peptide ntu hauv cod daim tawv nqaij FCP npaj los ntawm kev qhia enzymatic hydrolysis yog 82%, thiab transdermal absorption tus nqi yog 60% siab dua li cov khoom enzymatic.

Membrane sib cais tshuab: graded ntsuam xyuas, meej tswj ntawm molecular hnyav

Using ultrafiltration membranes (molecular weight cut-off of 500Da, 1000Da, 3000Da) to perform graded filtration of enzymatic hydrolysis products, removing large molecule impurities and ineffective peptide segments of ultra small molecules, and accurately enriching active peptide segments of 500-1000Da. The FCP purified by membrane separation has a molecular weight distribution uniformity (PDI) of ≤ 1.3, an active sequence retention rate of>95%, thiab txhim kho kev hloov pauv tau zoo.